335 research outputs found
Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018
Dataset: Trichodesmium field metaproteomes - single colony metaproteomesSingle colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/786694Gordon and Betty Moore Foundation (GBMF) GBMF3934, Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) GBMF3782, NSF Division of Ocean Sciences (NSF OCE) OCE-1657766, NSF Division of Ocean Sciences (NSF OCE) OCE-185071
The biogeochemistry of cobalt in the Sargasso Sea
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2001Processes that enable marine phytoplankton to acquire trace metals are fundamental to
our understanding of primary productivity and global carbon cycling. This thesis
explored the biogeochemistry of cobalt using analytical chemistry and physiological
experiments with the dominant phytoplankton species, Prochlorococcus.
A high sensitivity method for Co speciation was developed using hanging mercury drop
cathodic stripping voltammetry. Dissolved Co at the Bermuda Atlantic Time Series
station (BATS) in the Sargasso Sea was bound by strong organic complexes with a
conditional stability constant of logK=16.3l0.9. A depth profile of Co at BATS revealed
a nutrient-like profile. Biweekly time series measurements of total cobalt near Bermuda
from the MITESS sampler were 0-47pM throughout 1999, and averaged 20±10pM in
1999. A transect of total cobalt from BATS to American coastal waters ranged from 19-
133pM and correlated negatively with salinity (r2=0.93), suggestive of coastal waters as
an input source.
Prochlorococcus strains MED4-Ax and SS120 showed an absolute requirement for Co,
despite replete Zn. 57Co uptake rates and growth rates were enhanced by additions of
filtered low Co cultures, suggesting that a ligand is present that facilitates Co uptake.
Bottle incubations from a Synechococcus bloom in the Pacific showed production of
425pM strong cobalt ligand. These and other lines of evidence support the hypothesis
that a cobalt ligand, or cobalophore, is involved in cobalt uptake.
Co-limited Prochlorococcus cultures exhibited an increase in the fraction of cells in G2
relative to other cell cycle stages during exponential growth, and the durations of this
stage increased with decreasing cobalt concentrations. This effect was not observed with
Fe, N, or P-limited cultures, suggestive of a specific biochemical function of cobalt that
would interfere with the late stages of the cell cycle.
The ligand Teta was explored as a means to induce cobalt limitation. The CoTeta
complex was not bioavailable to the Sargasso Sea microbial assemblage in short-term
experiments. Bottle incubations with Teta did not induce cobalt limitation of
Prochlorococcus. These results are consistent with the lower conditional stability
constant for CoTeta (logK=11.2l0.1) relative to natural cobalt ligands in seawater, and
with culture studies that suggest uptake of cobalt via strong organic ligands.The work in this thesis was supported by a grant from the National Science Foundation
(#OCE-9618729) for cyanobacteria metal interactions in the Sargasso Sea. I have been
funded through WHOI on an NSF coastal traineeship (#DGE-9454129) for my first year,
followed by an EP A STAR Graduate Fellowship for the subsequent years. Additional
funding was supplied by the WHOI Educational Endowment Funds and by the WHOI
Ditty Bag fund for part of the DNA/cell cycle work
FASTA file of sequences in Trichodesmium field metaproteomes mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes from samples collected in the Atlantic and Pacific Ocean between 2000 and 2018
Dataset: Trichodesmium field metaproteomes - sequence fastaFASTA file of sequences in Trichodesmium field metaproteomes analyzed by 2D LC-MS/MS mapped to a Trichodesmium metagenome (IMG ID 2821474806) plus cyanoGEBA species genomes (Shih et al, 2013). Samples were collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.
Related datasets:
Trichodesmium field metaproteomes - peptide spectral counts: https://www.bco-dmo.org/dataset/787168
Trichodesmium field metaproteomes - protein spectral counts: https://www.bco-dmo.org/dataset/787147
Trichodesmium sample provenance: https://www.bco-dmo.org/dataset/787093 - Sample provenance file, which includes sample locations, filter sizes
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/787181Gordon and Betty Moore Foundation (GBMF) GBMF3934, Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) GBMF3782, NSF Division of Ocean Sciences (NSF OCE) OCE-1657766, NSF Division of Ocean Sciences (NSF OCE) OCE-185071
Net tow metaproteoome of Trichodesmium species mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes in units of normalized peptide spectral counts from samples collected in the Atlantic and Pacific Ocean between 2000 and 2018
Dataset: Trichodesmium field metaproteomes - peptide spectral countsNet tow metaproteoome of Trichodesmium species mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes, analyzed by 2D LC-MS/MS in units of normalized peptide spectral counts. Samples were collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/787168Gordon and Betty Moore Foundation (GBMF) GBMF3934, Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) GBMF3782, NSF Division of Ocean Sciences (NSF OCE) OCE-1657766, NSF Division of Ocean Sciences (NSF OCE) OCE-185071
Production of cobalt binding ligands in a Synechococcus feature at the Costa Rica upwelling dome
Author Posting. © American Society of Limnology and Oceanography, 2005. This is the author's version of the work. It is posted here by permission of American Society of Limnology and Oceanography for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography 50 (2005): 279-290.The Costa Rica upwelling dome (CRD; ~8.67ºN and 90.6ºW) was characterized chemically for cobalt and nickel abundances and speciation, and biologically using cyanobacterial abundances and phylogeny. Total dissolved cobalt was 93 pmol L-1at 90 m depth and decreased in surface waters to 45 pmol L-1 at 10 m. Cobalt was 40% labile at 90 m, but was completely complexed by strong ligands at 10 m. A surface transect out of the dome showed decreasing total dissolved cobalt from 57 pmol L-1 to 12 pmol L-1. Detection window studies showed that natural cobalt ligand complexes have conditional stability constants greater than 1016.8, and that competition with nickel did not release cobalt bound to organic complexes, consistent with natural cobalt ligands being Co(III)-complexes. Synechococcus cell densities at the CRD are among the highest reported in nature, varying between 1.2 x 106 to 3.7 x 106 cells ml-1. Phylogenetic analysis using the 16S-23S rDNA internally transcribed spacer showed the majority of clones were related to Synechococcus strain MIT S9220, while the remaining subset form a novel group within the marine Synechococcus lineage. In a bottle incubation experiment chlorophyll increased with cobalt and iron additions relative to each element alone and the unamended control treatment. Cobalt speciation analysis of incubation experiments revealed large quantities of strong cobalt ligand complexes in the cobalt addition treatments (401 pmol L-1), whereas cobalt added to a 0.2 mm filtered control remained predominantly labile (387 pmol L-1), demonstrating that the Synechococcus-dominated community is a source of strong cobalt ligands.This research was funded by NSF OCE-9618729, OCE-0327225, and OCE-0220826
The Frictionless Data Package : data containerization for addressing big data challenges [poster]
Presented at AGU Ocean Sciences, 11 - 16 February 2018, Portland, ORAt the Biological and Chemical Oceanography Data Management Office (BCO-DMO) Big Data challenges have been steadily increasing. The sizes of data submissions have grown as instrumentation improves. Complex data types can sometimes be stored across different repositories . This signals a paradigm shift where data and information that is meant to be tightly-coupled and has traditionally been stored under the same roof is now distributed across repositories and data stores. For domain-specific repositories like BCO-DMO, a new mechanism for assembling data, metadata and supporting documentation is needed.
Traditionally, data repositories have relied on a human's involvement throughout discovery and access workflows. This human could assess fitness for purpose by reading loosely coupled, unstructured information from web pages and documentation. Distributed storage was something that could be communicated in text that a human could read and understand. However, as machines play larger roles in the process of discovery and access of data, distributed resources must be described and packaged in ways that fit into machine automated workflows of discovery and access for assessing fitness for purpose by the end-user. Once machines have recommended a data resource as relevant to an investigator's needs, the data should be easy to integrate into that investigator's toolkits for analysis and visualization.
BCO-DMO is exploring the idea of data containerization, or packaging data and related information for easier transport, interpretation, and use. Data containerization reduces not only the friction data repositories experience trying to describe complex data resources, but also for end-users trying to access data with their own toolkits. In researching the landscape of data containerization, the Frictionlessdata Data Package (http://frictionlessdata.io/) provides a number of valuable advantages over similar solutions. This presentation will focus on these advantages and how the Frictionlessdata Data Package addresses a number of real-world use cases faced for data discovery, access, analysis and visualization in the age of Big Data.NSF #1435578, NSF #163971
The Frictionless Data Package : data containerization for automated scientific workflows [poster]
Presented at the Fall AGU Meeting, New Orleans, LA, 11-15 December 2017As cross-disciplinary geoscience research increasingly relies on machines to discover and access data, one of the critical
questions facing data repositories is how data and supporting materials should be packaged for consumption.
Traditionally, data repositories have relied on a human's involvement throughout discovery and access workflows. This
human could assess fitness for purpose by reading loosely coupled, unstructured information from web pages and
documentation. In attempts to shorten the time to science and access data resources across may disciplines,
expectations for machines to mediate the process of discovery and access is challenging data repository infrastructure.
This challenge is to find ways to deliver data and information in ways that enable machines to make better decisions by
enabling them to understand the data and metadata of many data types. Additionally, once machines have
recommended a data resource as relevant to an investigator's needs, the data resource should be easy to integrate into
that investigator's toolkits for analysis and visualization.
The Biological and Chemical Oceanography Data Management Office (BCO-DMO) supports NSF-funded OCE and PLR
investigators with their project's data management needs. These needs involve a number of varying data types some of
which require multiple files with differing formats. Presently, BCO-DMO has described these data types and the
important relationships between the type's data files through human-readable documentation on web pages. For
machines directly accessing data files from BCO-DMO, this documentation could be overlooked and lead to
misinterpreting the data. Instead, BCO-DMO is exploring the idea of data containerization, or packaging data and related
information for easier transport, interpretation, and use. In researching the landscape of data containerization, the
Frictionlessdata Data Package (http://frictionlessdata.io/) provides a number of valuable advantages over similar
solutions. This presentation will focus on these advantages and how the Frictionlessdata Data Package addresses a
number of real-world use cases faced for data discovery, access, analysis and visualization.National Science Foundation Award #1435578, Award #163971
Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph, Pseudoalteromonas (BB2-AT2): multiple bacterioferritin copies enable significant Fe storage
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mazzotta, M. G., McIlvin, M. R., & Saito, M. A. Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph, Pseudoalteromonas (BB2-AT2): multiple bacterioferritin copies enable significant Fe storage. Metallomics, (2020), doi:10.1039/d0mt00034e.Fe is a critical nutrient to the marine biological pump, which is the process that exports photosynthetically fixed carbon in the upper ocean to the deep ocean. Fe limitation controls photosynthetic activity in major regions of the oceans, and the subsequent degradation of exported photosynthetic material is facilitated particularly by marine heterotrophic bacteria. Despite their importance in the carbon cycle and the scarcity of Fe in seawater, the Fe requirements, storage and cytosolic utilization of these marine heterotrophs has been less studied. Here, we characterized the Fe metallome of Pseudoalteromonas (BB2-AT2). We found that with two copies of bacterioferritin (Bfr), Pseudoalteromonas possesses substantial capacity for luxury uptake of Fe. Fe : C in the whole cell metallome was estimated (assuming C : P stoichiometry ∼51 : 1) to be between ∼83 μmol : mol Fe : C, ∼11 fold higher than prior marine bacteria surveys. Under these replete conditions, other major cytosolic Fe-associated proteins were observed including superoxide dismutase (SodA; with other metal SOD isoforms absent under Fe replete conditions) and catalase (KatG) involved in reactive oxygen stress mitigation and aconitase (AcnB), succinate dehydrogenase (FrdB) and cytochromes (QcrA and Cyt1) involved in respiration. With the aid of singular value decomposition (SVD), we were able to computationally attribute peaks within the metallome to specific metalloprotein contributors. A putative Fe complex TonB transporter associated with the closely related Alteromonas bacterium was found to be abundant within the Pacific Ocean mesopelagic environment. Despite the extreme scarcity of Fe in seawater, the marine heterotroph Pseudoalteromonas has expansive Fe storage capacity and utilization strategies, implying that within detritus and sinking particles environments, there is significant opportunity for Fe acquisition. Together these results imply an evolved dedication of marine Pseudoalteromonas to maintaining an Fe metalloproteome, likely due to its dependence on Fe-based respiratory metabolism.M. G. M. was supported by the Camille and Henry Dreyfus Foundation Environmental Chemistry Postdoctoral Fellowship. We thank Kay Bidle (Rutgers University) for providing a culture of Pseudoalteromonas (BB2-AT2). We also thank Dawn Moran (WHOI) and Noelle Held (WHOI-MIT) for culturing assistance. We appreciate the Captain and Crew of the R/V Kilo Moana, and the many involved in the METZYME expedition sampling efforts. Discussions with Kevin Waldron (Newcastle University), Alison Butler (University of California, Santa Barbara), Lauren Manck (Scripps Institution of Oceanography), Randie Bundy (University of Washington) and Jake Gebbie (WHOI) were much appreciated. Funding for this research was provided by the Gordon and Betty Moore Foundation (3782), and NSF-OCE 1658030, 1736599, 1657766, 1924554, 1850719, 1924554
Relative protein abundance from scaled and corrected exclusive peptide spectral counts from the ProteOMZ R/V Falkor expedition cruise FK160115 in the Pelagic central Pacific Ocean in 2016
Dataset: ProteOMZ Exclusive Peptide Level Spectral CountsRelative protein abundance from scaled and corrected exclusive peptide spectral counts from 20-1250 m in the water column (0.2-3 µm filter size fraction) from the ProteOMZ R/V Falkor expedition. There are a total of 107,579 unique peptide sequences from 56,543 protein groups (88,251 proteins). Exclusive spectral counts are provided per sample as are the full dataset scaled and normalized spectral counts. The protein distributions in this dataset highlight the microbial dynamics across biomes in the central Pacific Ocean. These data were submitted in Saunders et al. (2022).
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/868030Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) GBMF3782, Schmidt Ocean Institute (SOI) R/V Falkor 160115 SOI ProteOMZ Expeditio
- …